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primary human uterine fibroblasts  (ScienCell)

 
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    ScienCell primary human uterine fibroblasts
    Primary Human Uterine Fibroblasts, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+human+uterine+fibroblasts/primary+human+uterine+fibroblasts/pmc11779628-159-0-4
    Average 90 stars, based on 1 article reviews
    primary human uterine fibroblasts - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Cervical mucus in linked human Cervix and Vagina Chips modulates vaginal dysbiosis
    Article Snippet: basal channel was coated with collagen I (200 μg/mL) (Corning, USA) and poly-L-lysine (15 μg/mL) (ScienCell, Cat# 0403(. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were then seeded at a density of 1 × 10 6 cells/mL in the basal channel and human vaginal epithelial cells (Lifeline Cell Technology, cat. no. FC-0083; donors 05328) were seeded at a density of 3 × 10 6 cells/mL in the apical channel. .. Chips were incubated at 37 °C with 5% CO 2 under static conditions until the cells formed a uniform monolayer.

    Article Title: Modulation of dysbiotic vaginal complications by cervical mucus revealed in linked human vagina and cervix chips
    Article Snippet: basal channel was coated with collagen I (200 μg/mL) (Corning, USA) and poly-L-lysine (15 μg/mL) (ScienCell, Cat# 0403 ) . .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were then seeded at a density of 1 × 10 cells/mL in the basal channel and the human vaginal epithelial cells (Lifeline Cell Technology, cat. no. FC-0083; donors 05328) were seeded at a density of 3 × 106 cells/mL in the apical channel. .. The chips were incubated at 37°C with 5% CO2 under static conditions till the cells formed a uniform monolayer.

    Article Title: Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip.
    Article Snippet: expanded in 75-cm2 tissue-culture flasks using vaginal epithelium growth medium (Lifeline Cell Technology, cat. no. LL-0068) to 60-70% confluency. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were expanded in 75-cm2 tissue-culture flasks coated with poly-L-lysine (15 μg/mL, ScienCell Research Laboratories, cat. no. 2301) using fibroblast growth medium (ScienCell Research Laboratories, cat. no. 7040) to 60–70% confluency. .. In the current configuration, we utilized commercially available uterine fibroblasts due to limited availability of the primary human vaginal fibrobla

    Article Title: Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip
    Article Snippet: expanded in 75-cm 2 tissue-culture flasks using vaginal epithelium growth medium (Lifeline Cell Technology, cat. no. LL-0068) to 60-70% confluency. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were expanded in 75-cm 2 tissue-culture flasks coated with poly-L-lysine (15 μg/mL, ScienCell Research Laboratories, cat. no. 2301) using fibroblast growth medium (ScienCell Research Laboratories, cat. no. 7040) to 60–70% confluency. .. In the current configuration, we utilized commercially available uterine fibroblasts due to limited availability of the primary human vaginal fibrobla

    Membrane:

    Article Title: Cervical mucus in linked human Cervix and Vagina Chips modulates vaginal dysbiosis
    Article Snippet: basal channel was coated with collagen I (200 μg/mL) (Corning, USA) and poly-L-lysine (15 μg/mL) (ScienCell, Cat# 0403(. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were then seeded at a density of 1 × 10 6 cells/mL in the basal channel and human vaginal epithelial cells (Lifeline Cell Technology, cat. no. FC-0083; donors 05328) were seeded at a density of 3 × 10 6 cells/mL in the apical channel. .. Chips were incubated at 37 °C with 5% CO 2 under static conditions until the cells formed a uniform monolayer.

    Article Title: Modulation of dysbiotic vaginal complications by cervical mucus revealed in linked human vagina and cervix chips
    Article Snippet: basal channel was coated with collagen I (200 μg/mL) (Corning, USA) and poly-L-lysine (15 μg/mL) (ScienCell, Cat# 0403 ) . .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were then seeded at a density of 1 × 10 cells/mL in the basal channel and the human vaginal epithelial cells (Lifeline Cell Technology, cat. no. FC-0083; donors 05328) were seeded at a density of 3 × 106 cells/mL in the apical channel. .. The chips were incubated at 37°C with 5% CO2 under static conditions till the cells formed a uniform monolayer.

    Article Title: Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip.
    Article Snippet: expanded in 75-cm2 tissue-culture flasks using vaginal epithelium growth medium (Lifeline Cell Technology, cat. no. LL-0068) to 60-70% confluency. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were expanded in 75-cm2 tissue-culture flasks coated with poly-L-lysine (15 μg/mL, ScienCell Research Laboratories, cat. no. 2301) using fibroblast growth medium (ScienCell Research Laboratories, cat. no. 7040) to 60–70% confluency. .. In the current configuration, we utilized commercially available uterine fibroblasts due to limited availability of the primary human vaginal fibrobla

    Article Title: Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip
    Article Snippet: expanded in 75-cm 2 tissue-culture flasks using vaginal epithelium growth medium (Lifeline Cell Technology, cat. no. LL-0068) to 60-70% confluency. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were expanded in 75-cm 2 tissue-culture flasks coated with poly-L-lysine (15 μg/mL, ScienCell Research Laboratories, cat. no. 2301) using fibroblast growth medium (ScienCell Research Laboratories, cat. no. 7040) to 60–70% confluency. .. In the current configuration, we utilized commercially available uterine fibroblasts due to limited availability of the primary human vaginal fibrobla

    Immunofluorescence:

    Article Title: Cervical mucus in linked human Cervix and Vagina Chips modulates vaginal dysbiosis
    Article Snippet: basal channel was coated with collagen I (200 μg/mL) (Corning, USA) and poly-L-lysine (15 μg/mL) (ScienCell, Cat# 0403(. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were then seeded at a density of 1 × 10 6 cells/mL in the basal channel and human vaginal epithelial cells (Lifeline Cell Technology, cat. no. FC-0083; donors 05328) were seeded at a density of 3 × 10 6 cells/mL in the apical channel. .. Chips were incubated at 37 °C with 5% CO 2 under static conditions until the cells formed a uniform monolayer.

    Article Title: Modulation of dysbiotic vaginal complications by cervical mucus revealed in linked human vagina and cervix chips
    Article Snippet: basal channel was coated with collagen I (200 μg/mL) (Corning, USA) and poly-L-lysine (15 μg/mL) (ScienCell, Cat# 0403 ) . .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were then seeded at a density of 1 × 10 cells/mL in the basal channel and the human vaginal epithelial cells (Lifeline Cell Technology, cat. no. FC-0083; donors 05328) were seeded at a density of 3 × 106 cells/mL in the apical channel. .. The chips were incubated at 37°C with 5% CO2 under static conditions till the cells formed a uniform monolayer.

    Article Title: Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip.
    Article Snippet: expanded in 75-cm2 tissue-culture flasks using vaginal epithelium growth medium (Lifeline Cell Technology, cat. no. LL-0068) to 60-70% confluency. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were expanded in 75-cm2 tissue-culture flasks coated with poly-L-lysine (15 μg/mL, ScienCell Research Laboratories, cat. no. 2301) using fibroblast growth medium (ScienCell Research Laboratories, cat. no. 7040) to 60–70% confluency. .. In the current configuration, we utilized commercially available uterine fibroblasts due to limited availability of the primary human vaginal fibrobla

    Article Title: Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip
    Article Snippet: expanded in 75-cm 2 tissue-culture flasks using vaginal epithelium growth medium (Lifeline Cell Technology, cat. no. LL-0068) to 60-70% confluency. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were expanded in 75-cm 2 tissue-culture flasks coated with poly-L-lysine (15 μg/mL, ScienCell Research Laboratories, cat. no. 2301) using fibroblast growth medium (ScienCell Research Laboratories, cat. no. 7040) to 60–70% confluency. .. In the current configuration, we utilized commercially available uterine fibroblasts due to limited availability of the primary human vaginal fibrobla

    Staining:

    Article Title: Cervical mucus in linked human Cervix and Vagina Chips modulates vaginal dysbiosis
    Article Snippet: basal channel was coated with collagen I (200 μg/mL) (Corning, USA) and poly-L-lysine (15 μg/mL) (ScienCell, Cat# 0403(. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were then seeded at a density of 1 × 10 6 cells/mL in the basal channel and human vaginal epithelial cells (Lifeline Cell Technology, cat. no. FC-0083; donors 05328) were seeded at a density of 3 × 10 6 cells/mL in the apical channel. .. Chips were incubated at 37 °C with 5% CO 2 under static conditions until the cells formed a uniform monolayer.

    Article Title: Modulation of dysbiotic vaginal complications by cervical mucus revealed in linked human vagina and cervix chips
    Article Snippet: basal channel was coated with collagen I (200 μg/mL) (Corning, USA) and poly-L-lysine (15 μg/mL) (ScienCell, Cat# 0403 ) . .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were then seeded at a density of 1 × 10 cells/mL in the basal channel and the human vaginal epithelial cells (Lifeline Cell Technology, cat. no. FC-0083; donors 05328) were seeded at a density of 3 × 106 cells/mL in the apical channel. .. The chips were incubated at 37°C with 5% CO2 under static conditions till the cells formed a uniform monolayer.

    Article Title: Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip.
    Article Snippet: expanded in 75-cm2 tissue-culture flasks using vaginal epithelium growth medium (Lifeline Cell Technology, cat. no. LL-0068) to 60-70% confluency. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were expanded in 75-cm2 tissue-culture flasks coated with poly-L-lysine (15 μg/mL, ScienCell Research Laboratories, cat. no. 2301) using fibroblast growth medium (ScienCell Research Laboratories, cat. no. 7040) to 60–70% confluency. .. In the current configuration, we utilized commercially available uterine fibroblasts due to limited availability of the primary human vaginal fibrobla

    Article Title: Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip
    Article Snippet: expanded in 75-cm 2 tissue-culture flasks using vaginal epithelium growth medium (Lifeline Cell Technology, cat. no. LL-0068) to 60-70% confluency. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were expanded in 75-cm 2 tissue-culture flasks coated with poly-L-lysine (15 μg/mL, ScienCell Research Laboratories, cat. no. 2301) using fibroblast growth medium (ScienCell Research Laboratories, cat. no. 7040) to 60–70% confluency. .. In the current configuration, we utilized commercially available uterine fibroblasts due to limited availability of the primary human vaginal fibrobla

    Permeability:

    Article Title: Cervical mucus in linked human Cervix and Vagina Chips modulates vaginal dysbiosis
    Article Snippet: basal channel was coated with collagen I (200 μg/mL) (Corning, USA) and poly-L-lysine (15 μg/mL) (ScienCell, Cat# 0403(. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were then seeded at a density of 1 × 10 6 cells/mL in the basal channel and human vaginal epithelial cells (Lifeline Cell Technology, cat. no. FC-0083; donors 05328) were seeded at a density of 3 × 10 6 cells/mL in the apical channel. .. Chips were incubated at 37 °C with 5% CO 2 under static conditions until the cells formed a uniform monolayer.

    Article Title: Modulation of dysbiotic vaginal complications by cervical mucus revealed in linked human vagina and cervix chips
    Article Snippet: basal channel was coated with collagen I (200 μg/mL) (Corning, USA) and poly-L-lysine (15 μg/mL) (ScienCell, Cat# 0403 ) . .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were then seeded at a density of 1 × 10 cells/mL in the basal channel and the human vaginal epithelial cells (Lifeline Cell Technology, cat. no. FC-0083; donors 05328) were seeded at a density of 3 × 106 cells/mL in the apical channel. .. The chips were incubated at 37°C with 5% CO2 under static conditions till the cells formed a uniform monolayer.

    Article Title: Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip.
    Article Snippet: expanded in 75-cm2 tissue-culture flasks using vaginal epithelium growth medium (Lifeline Cell Technology, cat. no. LL-0068) to 60-70% confluency. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were expanded in 75-cm2 tissue-culture flasks coated with poly-L-lysine (15 μg/mL, ScienCell Research Laboratories, cat. no. 2301) using fibroblast growth medium (ScienCell Research Laboratories, cat. no. 7040) to 60–70% confluency. .. In the current configuration, we utilized commercially available uterine fibroblasts due to limited availability of the primary human vaginal fibrobla

    Article Title: Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip
    Article Snippet: expanded in 75-cm 2 tissue-culture flasks using vaginal epithelium growth medium (Lifeline Cell Technology, cat. no. LL-0068) to 60-70% confluency. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were expanded in 75-cm 2 tissue-culture flasks coated with poly-L-lysine (15 μg/mL, ScienCell Research Laboratories, cat. no. 2301) using fibroblast growth medium (ScienCell Research Laboratories, cat. no. 7040) to 60–70% confluency. .. In the current configuration, we utilized commercially available uterine fibroblasts due to limited availability of the primary human vaginal fibrobla

    Quantitative RT-PCR:

    Article Title: Cervical mucus in linked human Cervix and Vagina Chips modulates vaginal dysbiosis
    Article Snippet: basal channel was coated with collagen I (200 μg/mL) (Corning, USA) and poly-L-lysine (15 μg/mL) (ScienCell, Cat# 0403(. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were then seeded at a density of 1 × 10 6 cells/mL in the basal channel and human vaginal epithelial cells (Lifeline Cell Technology, cat. no. FC-0083; donors 05328) were seeded at a density of 3 × 10 6 cells/mL in the apical channel. .. Chips were incubated at 37 °C with 5% CO 2 under static conditions until the cells formed a uniform monolayer.

    Article Title: Modulation of dysbiotic vaginal complications by cervical mucus revealed in linked human vagina and cervix chips
    Article Snippet: basal channel was coated with collagen I (200 μg/mL) (Corning, USA) and poly-L-lysine (15 μg/mL) (ScienCell, Cat# 0403 ) . .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were then seeded at a density of 1 × 10 cells/mL in the basal channel and the human vaginal epithelial cells (Lifeline Cell Technology, cat. no. FC-0083; donors 05328) were seeded at a density of 3 × 106 cells/mL in the apical channel. .. The chips were incubated at 37°C with 5% CO2 under static conditions till the cells formed a uniform monolayer.

    Article Title: Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip.
    Article Snippet: expanded in 75-cm2 tissue-culture flasks using vaginal epithelium growth medium (Lifeline Cell Technology, cat. no. LL-0068) to 60-70% confluency. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were expanded in 75-cm2 tissue-culture flasks coated with poly-L-lysine (15 μg/mL, ScienCell Research Laboratories, cat. no. 2301) using fibroblast growth medium (ScienCell Research Laboratories, cat. no. 7040) to 60–70% confluency. .. In the current configuration, we utilized commercially available uterine fibroblasts due to limited availability of the primary human vaginal fibrobla

    Article Title: Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip
    Article Snippet: expanded in 75-cm 2 tissue-culture flasks using vaginal epithelium growth medium (Lifeline Cell Technology, cat. no. LL-0068) to 60-70% confluency. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were expanded in 75-cm 2 tissue-culture flasks coated with poly-L-lysine (15 μg/mL, ScienCell Research Laboratories, cat. no. 2301) using fibroblast growth medium (ScienCell Research Laboratories, cat. no. 7040) to 60–70% confluency. .. In the current configuration, we utilized commercially available uterine fibroblasts due to limited availability of the primary human vaginal fibrobla

    Expressing:

    Article Title: Cervical mucus in linked human Cervix and Vagina Chips modulates vaginal dysbiosis
    Article Snippet: basal channel was coated with collagen I (200 μg/mL) (Corning, USA) and poly-L-lysine (15 μg/mL) (ScienCell, Cat# 0403(. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were then seeded at a density of 1 × 10 6 cells/mL in the basal channel and human vaginal epithelial cells (Lifeline Cell Technology, cat. no. FC-0083; donors 05328) were seeded at a density of 3 × 10 6 cells/mL in the apical channel. .. Chips were incubated at 37 °C with 5% CO 2 under static conditions until the cells formed a uniform monolayer.

    Article Title: Modulation of dysbiotic vaginal complications by cervical mucus revealed in linked human vagina and cervix chips
    Article Snippet: basal channel was coated with collagen I (200 μg/mL) (Corning, USA) and poly-L-lysine (15 μg/mL) (ScienCell, Cat# 0403 ) . .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were then seeded at a density of 1 × 10 cells/mL in the basal channel and the human vaginal epithelial cells (Lifeline Cell Technology, cat. no. FC-0083; donors 05328) were seeded at a density of 3 × 106 cells/mL in the apical channel. .. The chips were incubated at 37°C with 5% CO2 under static conditions till the cells formed a uniform monolayer.

    Article Title: Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip.
    Article Snippet: expanded in 75-cm2 tissue-culture flasks using vaginal epithelium growth medium (Lifeline Cell Technology, cat. no. LL-0068) to 60-70% confluency. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were expanded in 75-cm2 tissue-culture flasks coated with poly-L-lysine (15 μg/mL, ScienCell Research Laboratories, cat. no. 2301) using fibroblast growth medium (ScienCell Research Laboratories, cat. no. 7040) to 60–70% confluency. .. In the current configuration, we utilized commercially available uterine fibroblasts due to limited availability of the primary human vaginal fibrobla

    Article Title: Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip
    Article Snippet: expanded in 75-cm 2 tissue-culture flasks using vaginal epithelium growth medium (Lifeline Cell Technology, cat. no. LL-0068) to 60-70% confluency. .. Primary human uterine fibroblasts (ScienCell Research Laboratories, cat. no. 7040) were expanded in 75-cm 2 tissue-culture flasks coated with poly-L-lysine (15 μg/mL, ScienCell Research Laboratories, cat. no. 2301) using fibroblast growth medium (ScienCell Research Laboratories, cat. no. 7040) to 60–70% confluency. .. In the current configuration, we utilized commercially available uterine fibroblasts due to limited availability of the primary human vaginal fibrobla



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    Image Search Results


    a Schematic illustrating the female reproductive system including the uterus with the endometrium and myometrium, the ovary and fallopian tubes. Inset shows endometrial stromal cells embedded in extracellular matrix (ECM) containing a variety of proteins (e.g., laminin, collagens and fibronectin). b Schematic illustrating the relative levels of estrogen and progesterone during the menstrual cycle. c Schematic illustrating the endometrial tissue during the proliferative and remodeling phase of the menstrual cycle.

    Journal: bioRxiv

    Article Title: Mechanical Cues Regulate Estrogen and Progesterone-Induced Nascent ECM Deposition by Human Endometrial Stromal Cells

    doi: 10.1101/2025.10.14.682403

    Figure Lengend Snippet: a Schematic illustrating the female reproductive system including the uterus with the endometrium and myometrium, the ovary and fallopian tubes. Inset shows endometrial stromal cells embedded in extracellular matrix (ECM) containing a variety of proteins (e.g., laminin, collagens and fibronectin). b Schematic illustrating the relative levels of estrogen and progesterone during the menstrual cycle. c Schematic illustrating the endometrial tissue during the proliferative and remodeling phase of the menstrual cycle.

    Article Snippet: Primary endometrial stromal cells (PCS-460-010, ATCC; 25-year-old donor) were cultured in fibroblast growth media, containing 10% fetal bovine serum (PCS-201-030 and PCS-201-041, ATCC), and passaged twice prior cryopreservation.

    Techniques:

    a Schematic illustrating the structure of progesterone, estrogen, and 3’,5’-cyclic adenosine monophosphate (cAMP). b Timeline of endometrial stromal cell seeding on glass (-24 hours) and culture for 24 hours to reach confluency before an additional culture up to 96 hours without hormones (Ctrl) or with hormones/cAMP (+Hrm). Cells were fixed at 0, 24, 48, 72, and 96 hours for image analysis. c Schematic illustrating the change in endometrial stromal cell morphology in response to hormonal treatment leading to the secretion of hormones, such as prolactin. d Representative fluorescent images of Phalloidin (F-actin) and Hoechst (nucleus) of endometrial stromal cells cultured for 0, 24, and 48 hours without hormones (Ctrl, scale bars = 100 µm, insets = 55 µm). e Quantification of size and number of nuclei of endometrial stromal cells cultured for 0, 24, and 48 hours in control media (n = 3 replicates, 5 images per replicate from one representative experiment, **p≤0.001, **** p≤0.0001, ns = not significant by one-way ANOVA). f Representative fluorescent images of Phalloidin (F-actin) and Hoechst (nucleus) of endometrial stromal cells cultured for 0, 24, and 48 hours with hormones (+Hrm, scale bars = 100µm, inset = 55µm). g Quantification of size and number of nuclei of endometrial stromal cells cultured for 0, 24, and 48 hours in hormone media (n = 3 replicates, 5 images per replicate from one representative experiment, **p≤0.001, **** p≤0.0001, ns = not significant by one-way ANOVA).

    Journal: bioRxiv

    Article Title: Mechanical Cues Regulate Estrogen and Progesterone-Induced Nascent ECM Deposition by Human Endometrial Stromal Cells

    doi: 10.1101/2025.10.14.682403

    Figure Lengend Snippet: a Schematic illustrating the structure of progesterone, estrogen, and 3’,5’-cyclic adenosine monophosphate (cAMP). b Timeline of endometrial stromal cell seeding on glass (-24 hours) and culture for 24 hours to reach confluency before an additional culture up to 96 hours without hormones (Ctrl) or with hormones/cAMP (+Hrm). Cells were fixed at 0, 24, 48, 72, and 96 hours for image analysis. c Schematic illustrating the change in endometrial stromal cell morphology in response to hormonal treatment leading to the secretion of hormones, such as prolactin. d Representative fluorescent images of Phalloidin (F-actin) and Hoechst (nucleus) of endometrial stromal cells cultured for 0, 24, and 48 hours without hormones (Ctrl, scale bars = 100 µm, insets = 55 µm). e Quantification of size and number of nuclei of endometrial stromal cells cultured for 0, 24, and 48 hours in control media (n = 3 replicates, 5 images per replicate from one representative experiment, **p≤0.001, **** p≤0.0001, ns = not significant by one-way ANOVA). f Representative fluorescent images of Phalloidin (F-actin) and Hoechst (nucleus) of endometrial stromal cells cultured for 0, 24, and 48 hours with hormones (+Hrm, scale bars = 100µm, inset = 55µm). g Quantification of size and number of nuclei of endometrial stromal cells cultured for 0, 24, and 48 hours in hormone media (n = 3 replicates, 5 images per replicate from one representative experiment, **p≤0.001, **** p≤0.0001, ns = not significant by one-way ANOVA).

    Article Snippet: Primary endometrial stromal cells (PCS-460-010, ATCC; 25-year-old donor) were cultured in fibroblast growth media, containing 10% fetal bovine serum (PCS-201-030 and PCS-201-041, ATCC), and passaged twice prior cryopreservation.

    Techniques: Cell Culture, Control

    a Timeline of endometrial stromal cell seeding: culture for 24 hours to attach to the substrate before additional culture for 48 hours without hormones (Ctrl) or with hormones/cAMP (+Hrm) prior harvesting for image analysis. b Schematic illustrating norbornene-modified hyaluronic acid (NorHA) hydrogels with different elastic moduli using 1.29 (low) or 3.24 mM (high) dithiol. c Quantification of compressive Young’s modulus of 1.29 mM (low) and 3.24 mM (high) DTT crosslinked NorHA hydrogels (n = 3 hydrogels, ***p=0.0001 by Student’s t -test). d Representative fluorescent images of endometrial stromal cells cultured for 48 hours atop 5 kPa and 15 kPa hydrogels without hormones (Ctrl, scale bars = 100µm, inset = 55µm). e Quantification of number of nuclei and area of nascent ECM deposition, normalized to number of nuclei, of endometrial stromal cells cultured for 48 hours atop 5 kP and 15 kPa hydrogels (5kPa: n = 48 images from 4 independent experiments, 15 kPa: n = 40 images from 3 independent experiments, *p≤0.05, ns = not significant by Students t -test). f Representative fluorescent images and quantification of number of nuclei and area of nascent ECM, normalized to number of nuclei, deposited by endometrial stromal cells cultured for 48 hours atop 5 kPa hydrogels with hormones (+Hrm, scale bars = 100 µm, insets = 55 µm, n = 48 images from 4 independent experiments, ****p≤0.0001 by Students t -test). g Representative fluorescent images and quantification of number of nuclei and area of nascent ECM, normalized to number of nuclei, deposited by endometrial stromal cells cultured for 48 hours atop 15 kPa hydrogels with hormones (+Hrm, scale bars = 100 µm, insets = 55 µm, n = 38 images from 3 independent experiments, *p ≤ 0.05 by Students t -test).

    Journal: bioRxiv

    Article Title: Mechanical Cues Regulate Estrogen and Progesterone-Induced Nascent ECM Deposition by Human Endometrial Stromal Cells

    doi: 10.1101/2025.10.14.682403

    Figure Lengend Snippet: a Timeline of endometrial stromal cell seeding: culture for 24 hours to attach to the substrate before additional culture for 48 hours without hormones (Ctrl) or with hormones/cAMP (+Hrm) prior harvesting for image analysis. b Schematic illustrating norbornene-modified hyaluronic acid (NorHA) hydrogels with different elastic moduli using 1.29 (low) or 3.24 mM (high) dithiol. c Quantification of compressive Young’s modulus of 1.29 mM (low) and 3.24 mM (high) DTT crosslinked NorHA hydrogels (n = 3 hydrogels, ***p=0.0001 by Student’s t -test). d Representative fluorescent images of endometrial stromal cells cultured for 48 hours atop 5 kPa and 15 kPa hydrogels without hormones (Ctrl, scale bars = 100µm, inset = 55µm). e Quantification of number of nuclei and area of nascent ECM deposition, normalized to number of nuclei, of endometrial stromal cells cultured for 48 hours atop 5 kP and 15 kPa hydrogels (5kPa: n = 48 images from 4 independent experiments, 15 kPa: n = 40 images from 3 independent experiments, *p≤0.05, ns = not significant by Students t -test). f Representative fluorescent images and quantification of number of nuclei and area of nascent ECM, normalized to number of nuclei, deposited by endometrial stromal cells cultured for 48 hours atop 5 kPa hydrogels with hormones (+Hrm, scale bars = 100 µm, insets = 55 µm, n = 48 images from 4 independent experiments, ****p≤0.0001 by Students t -test). g Representative fluorescent images and quantification of number of nuclei and area of nascent ECM, normalized to number of nuclei, deposited by endometrial stromal cells cultured for 48 hours atop 15 kPa hydrogels with hormones (+Hrm, scale bars = 100 µm, insets = 55 µm, n = 38 images from 3 independent experiments, *p ≤ 0.05 by Students t -test).

    Article Snippet: Primary endometrial stromal cells (PCS-460-010, ATCC; 25-year-old donor) were cultured in fibroblast growth media, containing 10% fetal bovine serum (PCS-201-030 and PCS-201-041, ATCC), and passaged twice prior cryopreservation.

    Techniques: Modification, Cell Culture

    a Representative fluorescent images of cells seeded on 5 kPa gels without (Ctrl) and with hormones (+Hrm), stained with Hoechst (nuclei), Phalloidin (F-actin), DBCO-488 (nascent ECM), and anti-Fibronectin antibody (scale bars = 100 µm, insets = 55 µm). b Quantification of area of fibronectin deposited normalized to number of endometrial stromal cells cultured on 5 kPa hydrogels for 48 hours with and without hormones (5 kPa gels: n = 28 images from 3 independent experiments, ****p≤0.0001 by Student’s t -test). c Representative fluorescent images of cells seeded on 15 kPa gels without and with hormones, stained with Hoechst, Phalloidin, DBCO-488, and anti-Fibronectin antibody (scale bars = 100 µm, insets = 55 µm). d Quantification of area of fibronectin deposited normalized to number of endometrial stromal cells cultured on 15 kPa hydrogels for 48 hours with and without hormones (15 kPa gels: n = 19 images from 2 independent experiments, **p ≤ 0.01 by Student’s t -test). e Representative fluorescent images of cells seeded on glass coverslips without and with hormones, stained with Hoechst, Phalloidin, DBCO-488, and anti-Fibronectin antibody (scale bars = 100 µm, insets = 55 µm). f Quantification of area of fibronectin deposited normalized to number of endometrial stromal cells cultured on glass coverslips for 48 hours with and without hormones (glass: n = 15 images from one representative experiment, ns = not significant by Student’s t -test).

    Journal: bioRxiv

    Article Title: Mechanical Cues Regulate Estrogen and Progesterone-Induced Nascent ECM Deposition by Human Endometrial Stromal Cells

    doi: 10.1101/2025.10.14.682403

    Figure Lengend Snippet: a Representative fluorescent images of cells seeded on 5 kPa gels without (Ctrl) and with hormones (+Hrm), stained with Hoechst (nuclei), Phalloidin (F-actin), DBCO-488 (nascent ECM), and anti-Fibronectin antibody (scale bars = 100 µm, insets = 55 µm). b Quantification of area of fibronectin deposited normalized to number of endometrial stromal cells cultured on 5 kPa hydrogels for 48 hours with and without hormones (5 kPa gels: n = 28 images from 3 independent experiments, ****p≤0.0001 by Student’s t -test). c Representative fluorescent images of cells seeded on 15 kPa gels without and with hormones, stained with Hoechst, Phalloidin, DBCO-488, and anti-Fibronectin antibody (scale bars = 100 µm, insets = 55 µm). d Quantification of area of fibronectin deposited normalized to number of endometrial stromal cells cultured on 15 kPa hydrogels for 48 hours with and without hormones (15 kPa gels: n = 19 images from 2 independent experiments, **p ≤ 0.01 by Student’s t -test). e Representative fluorescent images of cells seeded on glass coverslips without and with hormones, stained with Hoechst, Phalloidin, DBCO-488, and anti-Fibronectin antibody (scale bars = 100 µm, insets = 55 µm). f Quantification of area of fibronectin deposited normalized to number of endometrial stromal cells cultured on glass coverslips for 48 hours with and without hormones (glass: n = 15 images from one representative experiment, ns = not significant by Student’s t -test).

    Article Snippet: Primary endometrial stromal cells (PCS-460-010, ATCC; 25-year-old donor) were cultured in fibroblast growth media, containing 10% fetal bovine serum (PCS-201-030 and PCS-201-041, ATCC), and passaged twice prior cryopreservation.

    Techniques: Staining, Cell Culture